Cargando…

Production of a recombinant polyester-cleaving hydrolase from Thermobifida fusca in Escherichia coli

The hydrolase (Thermobifida fusca hydrolase; TfH) from T. fusca was produced in Escherichia coli as fusion protein using the OmpA leader sequence and a His(6) tag. Productivity could be raised more than 100-fold. Both batch and fed-batch cultivations yield comparable cell specific productivities whe...

Descripción completa

Detalles Bibliográficos
Autores principales: Dresler, Karolin, van den Heuvel, Joop, Müller, Rolf-Joachim, Deckwer, Wolf-Dieter
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1705536/
https://www.ncbi.nlm.nih.gov/pubmed/16770590
http://dx.doi.org/10.1007/s00449-006-0069-9