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Production of a recombinant polyester-cleaving hydrolase from Thermobifida fusca in Escherichia coli

The hydrolase (Thermobifida fusca hydrolase; TfH) from T. fusca was produced in Escherichia coli as fusion protein using the OmpA leader sequence and a His(6) tag. Productivity could be raised more than 100-fold. Both batch and fed-batch cultivations yield comparable cell specific productivities whe...

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Detalles Bibliográficos
Autores principales:	Dresler, Karolin, van den Heuvel, Joop, Müller, Rolf-Joachim, Deckwer, Wolf-Dieter
Formato:	Texto
Lenguaje:	English
Publicado:	Springer-Verlag 2006
Materias:	Original Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1705536/ https://www.ncbi.nlm.nih.gov/pubmed/16770590 http://dx.doi.org/10.1007/s00449-006-0069-9

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