Control of carry-over contamination for PCR-based DNA methylation quantification using bisulfite treated DNA

In this study, we adapted the well known uracil DNA glycosylase (UNG) carry-over prevention system for PCR, and applied it to the analysis of DNA methylation based on sodium bisulfite conversion. As sodium bisulfite treatment converts unmethylated cytosine bases into uracil residues, bisulfite treat...

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Detalles Bibliográficos
Autores principales: Tetzner, Reimo, Dietrich, Dimo, Distler, Juergen
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1747185/
https://www.ncbi.nlm.nih.gov/pubmed/17135186
http://dx.doi.org/10.1093/nar/gkl955

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1747185/
https://www.ncbi.nlm.nih.gov/pubmed/17135186
http://dx.doi.org/10.1093/nar/gkl955

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