Cargando…

Control of carry-over contamination for PCR-based DNA methylation quantification using bisulfite treated DNA

In this study, we adapted the well known uracil DNA glycosylase (UNG) carry-over prevention system for PCR, and applied it to the analysis of DNA methylation based on sodium bisulfite conversion. As sodium bisulfite treatment converts unmethylated cytosine bases into uracil residues, bisulfite treat...

Descripción completa

Detalles Bibliográficos
Autores principales:	Tetzner, Reimo, Dietrich, Dimo, Distler, Juergen
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2007
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1747185/ https://www.ncbi.nlm.nih.gov/pubmed/17135186 http://dx.doi.org/10.1093/nar/gkl955

Ejemplares similares

Rapid quantification of DNA methylation through dNMP analysis following bisulfite-PCR
por: Yang, Inchul, et al.
Publicado: (2006)

Accurate quantification of DNA methylation using combined bisulfite restriction analysis coupled with the Agilent 2100 Bioanalyzer platform
por: Brena, Romulo Martin, et al.
Publicado: (2006)

Quantification of DNA methylation independent of sodium bisulfite conversion using methylation‐sensitive restriction enzymes and digital PCR
por: Nell, Rogier J., et al.
Publicado: (2020)

In-solution hybrid capture of bisulfite-converted DNA for targeted bisulfite sequencing of 174 ADME genes
por: Ivanov, Maxim, et al.
Publicado: (2013)

A ligation assay for multiplex analysis of CpG methylation using bisulfite-treated DNA
por: Dahl, Christina, et al.
Publicado: (2007)

A new method to prevent carry-over contaminations in two-step PCR NGS library preparations
por: Seitz, Volkhard, et al.
Publicado: (2015)

Single-cell, locus-specific bisulfite sequencing (SLBS) for direct detection of epimutations in DNA methylation patterns
por: Gravina, Silvia, et al.
Publicado: (2015)

Bisulfite sequencing Data Presentation and Compilation (BDPC) web server—a useful tool for DNA methylation analysis
por: Rohde, Christian, et al.
Publicado: (2008)

RNA cytosine methylation analysis by bisulfite sequencing
por: Schaefer, Matthias, et al.
Publicado: (2009)

Errors in the bisulfite conversion of DNA: modulating inappropriate- and failed-conversion frequencies
por: Genereux, Diane P., et al.
Publicado: (2008)

A mostly traditional approach improves alignment of bisulfite-converted DNA
por: Frith, Martin C., et al.
Publicado: (2012)

Accurate quantification of DNA using on-site PCR (osPCR) by characterizing DNA amplification at single-molecule resolution
por: Ding, Ruihua, et al.
Publicado: (2023)

Highly efficient single-stranded DNA ligation technique improves low-input whole-genome bisulfite sequencing by post-bisulfite adaptor tagging
por: Miura, Fumihito, et al.
Publicado: (2019)

A new method for accurate assessment of DNA quality after bisulfite treatment
por: Ehrich, Mathias, et al.
Publicado: (2007)

A simple algorithm for quantifying DNA methylation levels on multiple independent CpG sites in bisulfite genomic sequencing electropherograms
por: Leakey, Tatiana I., et al.
Publicado: (2008)

MSRE-PCR for analysis of gene-specific DNA methylation
por: Melnikov, Anatoliy A., et al.
Publicado: (2005)

BiSearch: primer-design and search tool for PCR on bisulfite-treated genomes
por: Tusnády, Gábor E., et al.
Publicado: (2005)

Laser capture microdissection–reduced representation bisulfite sequencing (LCM-RRBS) maps changes in DNA methylation associated with gonadectomy-induced adrenocortical neoplasia in the mouse
por: Schillebeeckx, Maximiliaan, et al.
Publicado: (2013)

Amplification-free whole-genome bisulfite sequencing by post-bisulfite adaptor tagging
por: Miura, Fumihito, et al.
Publicado: (2012)

A combined HM-PCR/SNuPE method for high sensitive detection of rare DNA methylation
por: Tierling, Sascha, et al.
Publicado: (2010)

Headloop suppression PCR and its application to selective amplification of methylated DNA sequences
por: Rand, Keith N., et al.
Publicado: (2005)

BatMeth: improved mapper for bisulfite sequencing reads on DNA methylation
por: Lim, Jing-Quan, et al.
Publicado: (2012)

Detection of differentially methylated regions from whole-genome bisulfite sequencing data without replicates
por: Wu, Hao, et al.
Publicado: (2015)

Determining subpopulation methylation profiles from bisulfite sequencing data of heterogeneous samples using DXM
por: Fong, Jerry, et al.
Publicado: (2021)

A polymerase engineered for bisulfite sequencing
por: Millar, Doug, et al.
Publicado: (2015)

Correction of PCR-bias in quantitative DNA methylation studies by means of cubic polynomial regression
por: Moskalev, Evgeny A., et al.
Publicado: (2011)

Base-resolution detection of N(4)-methylcytosine in genomic DNA using 4mC-Tet-assisted-bisulfite- sequencing
por: Yu, Miao, et al.
Publicado: (2015)

Bisulfite-independent analysis of CpG island methylation enables genome-scale stratification of single cells
por: Han, Lin, et al.
Publicado: (2017)

Bis-SNP: Combined DNA methylation and SNP calling for Bisulfite-seq data
por: Liu, Yaping, et al.
Publicado: (2012)

Novel method for high throughput DNA methylation marker evaluation using PNA-probe library hybridization and MALDI-TOF detection
por: Schatz, Philipp, et al.
Publicado: (2006)

Partial bisulfite conversion for unique template sequencing
por: Kumar, Vijay, et al.
Publicado: (2018)

MethylViewer: computational analysis and editing for bisulfite sequencing and methyltransferase accessibility protocol for individual templates (MAPit) projects
por: Pardo, Carolina E., et al.
Publicado: (2011)

DNA analysis with multiplex microarray-enhanced PCR
por: Pemov, A., et al.
Publicado: (2005)

LORD-Q: a long-run real-time PCR-based DNA-damage quantification method for nuclear and mitochondrial genome analysis
por: Lehle, Simon, et al.
Publicado: (2014)

Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA
por: Szwagierczak, Aleksandra, et al.
Publicado: (2010)

Quantification of DNA cleavage specificity in Hi-C experiments
por: Meluzzi, Dario, et al.
Publicado: (2016)

CpG_MPs: identification of CpG methylation patterns of genomic regions from high-throughput bisulfite sequencing data
por: Su, Jianzhong, et al.
Publicado: (2013)

Sensitive Melting Analysis after Real Time- Methylation Specific PCR (SMART-MSP): high-throughput and probe-free quantitative DNA methylation detection
por: Kristensen, Lasse S., et al.
Publicado: (2008)

Gel-free multiplexed reduced representation bisulfite sequencing for large-scale DNA methylation profiling
por: Boyle, Patrick, et al.
Publicado: (2012)

Combination of methylated-DNA precipitation and methylation-sensitive restriction enzymes (COMPARE-MS) for the rapid, sensitive and quantitative detection of DNA methylation
por: Yegnasubramanian, Srinivasan, et al.
Publicado: (2006)

Cannot write session to /tmp/vufind_sessions/sess_7u0h0aa808tfjbj2ibm0gbikdm