An improved method to identify BAC clones using pooled overgos

Hybridization using overgo probes is an established approach for screening arrayed bacterial artificial chromosome (BAC) libraries. We have improved the use of overgos by increasing the yield of positive clones using reduced levels of radioisotopes and enzyme. The strategy involves labeling with all...

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Detalles Bibliográficos
Autores principales: Madishetty, Kavitha, Condamine, Pascal, Svensson, Jan T., Rodriguez, Edmundo, Close, Timothy J.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1761434/
https://www.ncbi.nlm.nih.gov/pubmed/17151072
http://dx.doi.org/10.1093/nar/gkl920

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1761434/
https://www.ncbi.nlm.nih.gov/pubmed/17151072
http://dx.doi.org/10.1093/nar/gkl920

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