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Immunolocalization of phospho-S6 kinases: a new way to detect mitosis in tissue sections and in cell culture

During a study on the mTor pathway in the rat kidney we observed a striking increase of the phosphorylation of the S6 kinase in mitosis. In cryostat sections of perfusion-fixed tissue mitotic cells appeared as bright spots in immunofluorescence using an antibody specific for the phosphorylation site...

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Detalles Bibliográficos
Autores principales: Schmidt, Thomas, Wahl, Patricia, Wüthrich, Rudolf P., Vogetseder, Alexander, Picard, Nicolas, Kaissling, Brigitte, Le Hir, Michel
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1779630/
https://www.ncbi.nlm.nih.gov/pubmed/17136413
http://dx.doi.org/10.1007/s00418-006-0255-5