Degenerated primer design to amplify the heavy chain variable region from immunoglobulin cDNA

BACKGROUND: The amplification of variable regions of immunoglobulins has become a major challenge in the cloning of antibody genes, whether from hybridoma cell lines or splenic B cells. Using conventional protocols, the heavy-chain variable region genes often are not amplified successfully from the...

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Detalles Bibliográficos
Autores principales: Wang, Ying, Chen, Wei, Li, Xu, Cheng, Bing
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1780117/
https://www.ncbi.nlm.nih.gov/pubmed/17217527
http://dx.doi.org/10.1186/1471-2105-7-S4-S9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1780117/
https://www.ncbi.nlm.nih.gov/pubmed/17217527
http://dx.doi.org/10.1186/1471-2105-7-S4-S9

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