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Degenerated primer design to amplify the heavy chain variable region from immunoglobulin cDNA
BACKGROUND: The amplification of variable regions of immunoglobulins has become a major challenge in the cloning of antibody genes, whether from hybridoma cell lines or splenic B cells. Using conventional protocols, the heavy-chain variable region genes often are not amplified successfully from the...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2006
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1780117/ https://www.ncbi.nlm.nih.gov/pubmed/17217527 http://dx.doi.org/10.1186/1471-2105-7-S4-S9 |