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Acidosis activates complement system in vitro.

We investigated the in vitro effect of different forms of acidosis (pH 7.0) on the formation of anaphylatoxins C3a and C5a. Metabolic acidosis due to addition of hydrochloric acid (10 micromol/ml blood) or lactic acid (5.5 micromol/ml) to heparin blood (N=12) caused significant activation of C3a and...

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Detalles Bibliográficos
Autores principales: Emeis, M, Sonntag, J, Willam, C, Strauss, E, Walka, M M, Obladen, M
Formato: Texto
Lenguaje:English
Publicado: 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781874/
https://www.ncbi.nlm.nih.gov/pubmed/9927235
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author Emeis, M
Sonntag, J
Willam, C
Strauss, E
Walka, M M
Obladen, M
author_facet Emeis, M
Sonntag, J
Willam, C
Strauss, E
Walka, M M
Obladen, M
author_sort Emeis, M
collection PubMed
description We investigated the in vitro effect of different forms of acidosis (pH 7.0) on the formation of anaphylatoxins C3a and C5a. Metabolic acidosis due to addition of hydrochloric acid (10 micromol/ml blood) or lactic acid (5.5 micromol/ml) to heparin blood (N=12) caused significant activation of C3a and C5a compared to control (both p=0.002). Respiratory acidosis activated C3a (p=0.007) and C5a (p=0.003) compared to normocapnic controls. Making blood samples with lactic acidosis hypocapnic resulted in a median pH of 7.37. In this respiratory compensated metabolic acidosis, C3a and C5a were not increased. These experiments show that acidosis itself and not lactate trigger for activation of complement components C3 and C5.
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spelling pubmed-17818742007-01-25 Acidosis activates complement system in vitro. Emeis, M Sonntag, J Willam, C Strauss, E Walka, M M Obladen, M Mediators Inflamm Research Article We investigated the in vitro effect of different forms of acidosis (pH 7.0) on the formation of anaphylatoxins C3a and C5a. Metabolic acidosis due to addition of hydrochloric acid (10 micromol/ml blood) or lactic acid (5.5 micromol/ml) to heparin blood (N=12) caused significant activation of C3a and C5a compared to control (both p=0.002). Respiratory acidosis activated C3a (p=0.007) and C5a (p=0.003) compared to normocapnic controls. Making blood samples with lactic acidosis hypocapnic resulted in a median pH of 7.37. In this respiratory compensated metabolic acidosis, C3a and C5a were not increased. These experiments show that acidosis itself and not lactate trigger for activation of complement components C3 and C5. 1998 /pmc/articles/PMC1781874/ /pubmed/9927235 Text en
spellingShingle Research Article
Emeis, M
Sonntag, J
Willam, C
Strauss, E
Walka, M M
Obladen, M
Acidosis activates complement system in vitro.
title Acidosis activates complement system in vitro.
title_full Acidosis activates complement system in vitro.
title_fullStr Acidosis activates complement system in vitro.
title_full_unstemmed Acidosis activates complement system in vitro.
title_short Acidosis activates complement system in vitro.
title_sort acidosis activates complement system in vitro.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1781874/
https://www.ncbi.nlm.nih.gov/pubmed/9927235
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