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Construction and characterization of new piggyBac vectors for constitutive or inducible expression of heterologous gene pairs and the identification of a previously unrecognized activator sequence in piggyBac

BACKGROUND: We constructed and characterized several new piggyBac vectors to provide transposition of constitutively- or inducibly-expressible heterologous gene pairs. The dual constitutive control element consists of back-to-back copies of a baculovirus immediate early (ie1) promoter separated by a...

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Detalles Bibliográficos
Autores principales: Shi, Xianzong, Harrison, Robert L, Hollister, Jason R, Mohammed, Ahmed, Fraser, Malcolm J, Jarvis, Donald L
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1783651/
https://www.ncbi.nlm.nih.gov/pubmed/17233894
http://dx.doi.org/10.1186/1472-6750-7-5