Heating as a rapid purification method for recovering correctly-folded thermotolerant VH and VHH domains

BACKGROUND: Recombinant antibodies from Camelidae (VHHs) are potentially useful tools for both basic research and biotechnological applications because of their small size, robustness, easy handling and possibility to refold after chemio-physical denaturation. Their heat tolerance is a particularly...

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Detalles Bibliográficos
Autores principales: Olichon, Aurelien, Schweizer, Daniel, Muyldermans, Serge, de Marco, Ario
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1790891/
https://www.ncbi.nlm.nih.gov/pubmed/17257422
http://dx.doi.org/10.1186/1472-6750-7-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1790891/
https://www.ncbi.nlm.nih.gov/pubmed/17257422
http://dx.doi.org/10.1186/1472-6750-7-7

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