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Heating as a rapid purification method for recovering correctly-folded thermotolerant VH and VHH domains
BACKGROUND: Recombinant antibodies from Camelidae (VHHs) are potentially useful tools for both basic research and biotechnological applications because of their small size, robustness, easy handling and possibility to refold after chemio-physical denaturation. Their heat tolerance is a particularly...
Autores principales: | Olichon, Aurelien, Schweizer, Daniel, Muyldermans, Serge, de Marco, Ario |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1790891/ https://www.ncbi.nlm.nih.gov/pubmed/17257422 http://dx.doi.org/10.1186/1472-6750-7-7 |
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