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Lymphocytes from Enlarged Iliac Lymph Nodes as Fusion Partners for the Production of Monoclonal Antibodies after a Single Tail Base Immunization Attempt

A novel method of preparing hybridomas producing mouse monoclonal antibodies was ­established, called “the mouse iliac lymph node method”. Lymphocytes from enlarged iliac lymph nodes from mice injected intramuscularly at the tail base with an emulsion of antigen and Freund’s adjuvant were used for c...

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Detalles Bibliográficos
Autores principales: Sado, Yoshikazu, Inoue, Satoko, Tomono, Yasuko, Omori, Hiroyuki
Formato: Texto
Lenguaje:English
Publicado: Japan Society of Histochemistry and Cytochemistry 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1790974/
https://www.ncbi.nlm.nih.gov/pubmed/17327928
http://dx.doi.org/10.1267/ahc.06001