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Lymphocytes from Enlarged Iliac Lymph Nodes as Fusion Partners for the Production of Monoclonal Antibodies after a Single Tail Base Immunization Attempt
A novel method of preparing hybridomas producing mouse monoclonal antibodies was established, called “the mouse iliac lymph node method”. Lymphocytes from enlarged iliac lymph nodes from mice injected intramuscularly at the tail base with an emulsion of antigen and Freund’s adjuvant were used for c...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Japan Society of Histochemistry and Cytochemistry
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1790974/ https://www.ncbi.nlm.nih.gov/pubmed/17327928 http://dx.doi.org/10.1267/ahc.06001 |