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The Use of Coded PCR Primers Enables High-Throughput Sequencing of Multiple Homolog Amplification Products by 454 Parallel Sequencing

BACKGROUND: The invention of the Genome Sequence 20™ DNA Sequencing System (454 parallel sequencing platform) has enabled the rapid and high-volume production of sequence data. Until now, however, individual emulsion PCR (emPCR) reactions and subsequent sequencing runs have been unable to combine te...

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Detalles Bibliográficos
Autores principales: Binladen, Jonas, Gilbert, M. Thomas P., Bollback, Jonathan P., Panitz, Frank, Bendixen, Christian, Nielsen, Rasmus, Willerslev, Eske
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1797623/
https://www.ncbi.nlm.nih.gov/pubmed/17299583
http://dx.doi.org/10.1371/journal.pone.0000197