Elimination of laboratory ozone leads to a dramatic improvement in the reproducibility of microarray gene expression measurements

BACKGROUND: Environmental ozone can rapidly degrade cyanine 5 (Cy5), a fluorescent dye commonly used in microarray gene expression studies. Cyanine 3 (Cy3) is much less affected by atmospheric ozone. Degradation of the Cy5 signal relative to the Cy3 signal in 2-color microarrays will adversely reduc...

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Detalles Bibliográficos
Autores principales: Branham, William S, Melvin, Cathy D, Han, Tao, Desai, Varsha G, Moland, Carrie L, Scully, Adam T, Fuscoe, James C
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1800842/
https://www.ncbi.nlm.nih.gov/pubmed/17295919
http://dx.doi.org/10.1186/1472-6750-7-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1800842/
https://www.ncbi.nlm.nih.gov/pubmed/17295919
http://dx.doi.org/10.1186/1472-6750-7-8

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