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Elimination of laboratory ozone leads to a dramatic improvement in the reproducibility of microarray gene expression measurements
BACKGROUND: Environmental ozone can rapidly degrade cyanine 5 (Cy5), a fluorescent dye commonly used in microarray gene expression studies. Cyanine 3 (Cy3) is much less affected by atmospheric ozone. Degradation of the Cy5 signal relative to the Cy3 signal in 2-color microarrays will adversely reduc...
| Autores principales: | , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2007
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1800842/ https://www.ncbi.nlm.nih.gov/pubmed/17295919 http://dx.doi.org/10.1186/1472-6750-7-8 |