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Baculovirus-mediated gene transfer and recombinant protein expression do not interfere with insulin dependent phosphorylation of PKB/Akt in human SHSY-5Y and C3A cells

BACKGROUND: Recombinant adenovirus vectors and transfection agents comprising cationic lipids are widely used as gene delivery vehicles for functional expression in cultured cells. Consequently, these tools are utilized to investigate the effects of functional over-expression of proteins on insulin...

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Detalles Bibliográficos
Autores principales: Andersson, Monica, Warolén, Malin, Nilsson, Joakim, Selander, Martin, Sterky, Catharina, Bergdahl, Katrin, Sörving, Christina, James, Stephen R, Doverskog, Magnus
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1808450/
https://www.ncbi.nlm.nih.gov/pubmed/17309805
http://dx.doi.org/10.1186/1471-2121-8-6