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Preservation of large-scale chromatin structure in FISH experiments

The nuclear organization of specific endogenous chromatin regions can be investigated only by fluorescence in situ hybridization (FISH). One of the two fixation procedures is typically applied: (1) buffered formaldehyde or (2) hypotonic shock with methanol acetic acid fixation followed by dropping o...

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Detalles Bibliográficos
Autores principales: Hepperger, Claudia, Otten, Simone, von Hase, Johann, Dietzel, Steffen
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1824788/
https://www.ncbi.nlm.nih.gov/pubmed/17119992
http://dx.doi.org/10.1007/s00412-006-0084-2