High efficiency electrotransformation of Lactococcus lactis spp. lactis cells pretreated with lithium acetate and dithiothreitol

BACKGROUND: A goal for the food industry has always been to improve strains of Lactococcus lactis and stabilize beneficial traits. Genetic engineering is used extensively for manipulating this lactic acid bacterium, while electropolation is the most widely used technique for introducing foreign DNA...

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Detalles Bibliográficos
Autores principales: Papagianni, Maria, Avramidis, Nicholaos, Filioussis, George
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1838899/
https://www.ncbi.nlm.nih.gov/pubmed/17374174
http://dx.doi.org/10.1186/1472-6750-7-15

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1838899/
https://www.ncbi.nlm.nih.gov/pubmed/17374174
http://dx.doi.org/10.1186/1472-6750-7-15

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