A recombinase system facilitates cloning of expression cassettes in the ciliate Tetrahymena thermophila

BACKGROUND: Tetrahymena thermophila is one of the best characterized unicellular eukaryotes and its genome is sequenced in its entirety. However, the AT-richness of the genome and an unusual codon usage cause problems in cloning and expression of the ciliate DNA. To overcome these technical hiatuses...

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Detalles Bibliográficos
Autores principales: Weide, Thomas, Bockau, Ulrike, Rave, Angelika, Herrmann, Lutz, Hartmann, Marcus WW
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1839094/
https://www.ncbi.nlm.nih.gov/pubmed/17328820
http://dx.doi.org/10.1186/1471-2180-7-12

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1839094/
https://www.ncbi.nlm.nih.gov/pubmed/17328820
http://dx.doi.org/10.1186/1471-2180-7-12

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