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A recombinase system facilitates cloning of expression cassettes in the ciliate Tetrahymena thermophila
BACKGROUND: Tetrahymena thermophila is one of the best characterized unicellular eukaryotes and its genome is sequenced in its entirety. However, the AT-richness of the genome and an unusual codon usage cause problems in cloning and expression of the ciliate DNA. To overcome these technical hiatuses...
| Autores principales: | , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2007
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1839094/ https://www.ncbi.nlm.nih.gov/pubmed/17328820 http://dx.doi.org/10.1186/1471-2180-7-12 |