Improvement of a low pH antigen-antibody dissociation procedure for ELISA measurement of circulating anti-Aβ antibodies

BACKGROUND: Prior work from our group found that acid dissociation (pH 2.5 incubation) of serum from APP transgenic mice vaccinated against Aβ increased the apparent anti-Aβ titers, suggesting antibody masking by antigen in the ELISA assay. Subsequently, we found that pH 2.5 incubation of serum from...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Qingyou, Gordon, Marcia, Cao, Chuanhai, Ugen, Kenneth E, Morgan, Dave
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1845169/
https://www.ncbi.nlm.nih.gov/pubmed/17374155
http://dx.doi.org/10.1186/1471-2202-8-22

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1845169/
https://www.ncbi.nlm.nih.gov/pubmed/17374155
http://dx.doi.org/10.1186/1471-2202-8-22

Ejemplares similares