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Optimization of laser capture microdissection and RNA amplification for gene expression profiling of prostate cancer

BACKGROUND: To discover prostate cancer biomarkers, we profiled gene expression in benign and malignant cells laser capture microdissected (LCM) from prostate tissues and metastatic prostatic adenocarcinomas. Here we present methods developed, optimized, and validated to obtain high quality gene exp...

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Detalles Bibliográficos
Autores principales: Kube, Dagmar M, Savci-Heijink, Cemile D, Lamblin, Anne-Françoise, Kosari, Farhad, Vasmatzis, George, Cheville, John C, Connelly, Donald P, Klee, George G
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1847526/
https://www.ncbi.nlm.nih.gov/pubmed/17376245
http://dx.doi.org/10.1186/1471-2199-8-25