An inducible system for expression and validation of the specificity of short hairpin RNA in mammalian cells

RNA interference (RNAi) by means of short hairpin RNA (shRNA) has developed into a powerful tool for loss-of-function analysis in mammalian cells. The principal problem in RNAi experiments is off-target effects, and the most vigorous demonstration of the specificity of shRNA is the rescue of the RNA...

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Detalles Bibliográficos
Autores principales: Ma, Hoi Tang, On, Kin Fan, Tsang, Yiu Huen, Poon, Randy Y.C.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1851631/
https://www.ncbi.nlm.nih.gov/pubmed/17234679
http://dx.doi.org/10.1093/nar/gkl1109

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1851631/
https://www.ncbi.nlm.nih.gov/pubmed/17234679
http://dx.doi.org/10.1093/nar/gkl1109

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