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An inducible system for expression and validation of the specificity of short hairpin RNA in mammalian cells
RNA interference (RNAi) by means of short hairpin RNA (shRNA) has developed into a powerful tool for loss-of-function analysis in mammalian cells. The principal problem in RNAi experiments is off-target effects, and the most vigorous demonstration of the specificity of shRNA is the rescue of the RNA...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2007
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1851631/ https://www.ncbi.nlm.nih.gov/pubmed/17234679 http://dx.doi.org/10.1093/nar/gkl1109 |