Reversible site-specific tagging of enzymatically synthesized RNAs using aldehyde–hydrazine chemistry and protease-cleavable linkers

The investigation of RNA structure, dynamics and biological function often requires the site-specific incorporation of non-natural moieties. Here we describe the functionalization of RNA transcripts by aldehyde–hydrazine chemistry using a simple initiator nucleotide that carries an acetal-protected...

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Detalles Bibliográficos
Autores principales: Pfander, Stephanie, Fiammengo, Roberto, Kirin, Srećko I., Metzler-Nolte, Nils, Jäschke, Andres
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1851632/
https://www.ncbi.nlm.nih.gov/pubmed/17259220
http://dx.doi.org/10.1093/nar/gkl1110

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1851632/
https://www.ncbi.nlm.nih.gov/pubmed/17259220
http://dx.doi.org/10.1093/nar/gkl1110

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