The gateway pDEST17 expression vector encodes a −1 ribosomal frameshifting sequence

The attB1 site in the Gateway (Invitrogen) bacterial expression vector pDEST17, necessary for in vitro site-specific recombination, contains the sequence AAA-AAA. The sequence A-AAA-AAG within the Escherichia coli dnaX gene is recognized as ‘slippery’ and promotes −1 translational frameshifting. We...

Descripción completa

Detalles Bibliográficos
Autores principales: Belfield, Eric J., Hughes, Richard K., Tsesmetzis, Nicolas, Naldrett, Mike J., Casey, Rod
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1851650/
https://www.ncbi.nlm.nih.gov/pubmed/17272299
http://dx.doi.org/10.1093/nar/gkm003

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1851650/
https://www.ncbi.nlm.nih.gov/pubmed/17272299
http://dx.doi.org/10.1093/nar/gkm003

Ejemplares similares