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The gateway pDEST17 expression vector encodes a −1 ribosomal frameshifting sequence
The attB1 site in the Gateway (Invitrogen) bacterial expression vector pDEST17, necessary for in vitro site-specific recombination, contains the sequence AAA-AAA. The sequence A-AAA-AAG within the Escherichia coli dnaX gene is recognized as ‘slippery’ and promotes −1 translational frameshifting. We...
Autores principales: | Belfield, Eric J., Hughes, Richard K., Tsesmetzis, Nicolas, Naldrett, Mike J., Casey, Rod |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1851650/ https://www.ncbi.nlm.nih.gov/pubmed/17272299 http://dx.doi.org/10.1093/nar/gkm003 |
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