DNA supercoiling suppresses real-time PCR: a new approach to the quantification of mitochondrial DNA damage and repair

As a gold standard for quantification of starting amounts of nucleic acids, real-time PCR is increasingly used in quantitative analysis of mtDNA copy number in medical research. Using supercoiled plasmid DNA and mtDNA modified both in vitro and in cancer cells, we demonstrated that conformational ch...

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Detalles Bibliográficos
Autores principales: Chen, Jinsong, Kadlubar, Fred F., Chen, Junjian Z.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1851651/
https://www.ncbi.nlm.nih.gov/pubmed/17284464
http://dx.doi.org/10.1093/nar/gkm010

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1851651/
https://www.ncbi.nlm.nih.gov/pubmed/17284464
http://dx.doi.org/10.1093/nar/gkm010

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