RNA mutagenesis yields highly diverse mRNA libraries for in vitro protein evolution

BACKGROUND: In protein drug development, in vitro molecular optimization or protein maturation can be used to modify protein properties. One basic approach to protein maturation is the introduction of random DNA mutations into the target gene sequence to produce a library of variants that can be scr...

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Detalles Bibliográficos
Autores principales: Kopsidas, George, Carman, Rachael K, Stutt, Emma L, Raicevic, Anna, Roberts, Anthony S, Siomos, Mary-Anne V, Dobric, Nada, Pontes-Braz, Luisa, Coia, Greg
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1855321/
https://www.ncbi.nlm.nih.gov/pubmed/17425805
http://dx.doi.org/10.1186/1472-6750-7-18

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1855321/
https://www.ncbi.nlm.nih.gov/pubmed/17425805
http://dx.doi.org/10.1186/1472-6750-7-18

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