Novel DNA probes with low background and high hybridization-triggered fluorescence

Novel fluorogenic DNA probes are described. The probes (called Pleiades) have a minor groove binder (MGB) and a fluorophore at the 5′-end and a non-fluorescent quencher at the 3′-end of the DNA sequence. This configuration provides surprisingly low background and high hybridization-triggered fluores...

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Detalles Bibliográficos
Autores principales: Lukhtanov, Eugeny A., Lokhov, Sergey G., Gorn, Vladimir V., Podyminogin, Mikhail A., Mahoney, Walt
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1865069/
https://www.ncbi.nlm.nih.gov/pubmed/17259212
http://dx.doi.org/10.1093/nar/gkl1136

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1865069/
https://www.ncbi.nlm.nih.gov/pubmed/17259212
http://dx.doi.org/10.1093/nar/gkl1136

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