A versatile ligation-independent cloning method suitable for high-throughput expression screening applications

This article describes the construction of a set of versatile expression vectors based on the In-Fusion™ cloning enzyme and their use for high-throughput cloning and expression screening. Modifications to commonly used vectors rendering them compatible with In-Fusion™ has produced a ligation-indepen...

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Detalles Bibliográficos
Autores principales: Berrow, Nick S., Alderton, David, Sainsbury, Sarah, Nettleship, Joanne, Assenberg, Rene, Rahman, Nahid, Stuart, David I., Owens, Raymond J.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1874605/
https://www.ncbi.nlm.nih.gov/pubmed/17317681
http://dx.doi.org/10.1093/nar/gkm047

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1874605/
https://www.ncbi.nlm.nih.gov/pubmed/17317681
http://dx.doi.org/10.1093/nar/gkm047

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