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A versatile ligation-independent cloning method suitable for high-throughput expression screening applications
This article describes the construction of a set of versatile expression vectors based on the In-Fusion™ cloning enzyme and their use for high-throughput cloning and expression screening. Modifications to commonly used vectors rendering them compatible with In-Fusion™ has produced a ligation-indepen...
| Autores principales: | , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2007
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1874605/ https://www.ncbi.nlm.nih.gov/pubmed/17317681 http://dx.doi.org/10.1093/nar/gkm047 |