Mg(2+)-dependent folding of a Diels-Alderase ribozyme probed by single-molecule FRET analysis

Here, we report a single-molecule fluorescence resonance energy transfer (FRET) study of a Diels-Alderase (DAse) ribozyme, a 49-mer RNA with true catalytic properties. The DAse ribozyme was labeled with Cy3 and Cy5 as a FRET pair of dyes to observe intramolecular folding, which is a prerequisite for...

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Detalles Bibliográficos
Autores principales: Kobitski, Andrei Yu., Nierth, Alexander, Helm, Mark, Jäschke, Andres, Nienhaus, G. Ulrich
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1874616/
https://www.ncbi.nlm.nih.gov/pubmed/17344321
http://dx.doi.org/10.1093/nar/gkm072

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1874616/
https://www.ncbi.nlm.nih.gov/pubmed/17344321
http://dx.doi.org/10.1093/nar/gkm072

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