Cargando…

Mg(2+)-dependent folding of a Diels-Alderase ribozyme probed by single-molecule FRET analysis

Here, we report a single-molecule fluorescence resonance energy transfer (FRET) study of a Diels-Alderase (DAse) ribozyme, a 49-mer RNA with true catalytic properties. The DAse ribozyme was labeled with Cy3 and Cy5 as a FRET pair of dyes to observe intramolecular folding, which is a prerequisite for...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kobitski, Andrei Yu., Nierth, Alexander, Helm, Mark, Jäschke, Andres, Nienhaus, G. Ulrich
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2007
Materias:	RNA
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1874616/ https://www.ncbi.nlm.nih.gov/pubmed/17344321 http://dx.doi.org/10.1093/nar/gkm072

Ejemplares similares

Three critical hydrogen bonds determine the catalytic activity of the Diels–Alderase ribozyme
por: Kraut, Stefanie, et al.
Publicado: (2012)

Controlling the rate of organic reactions: rational design of allosteric Diels-Alderase ribozymes
por: Amontov, Sergey, et al.
Publicado: (2006)

Complex RNA Folding Kinetics Revealed by Single-Molecule FRET and Hidden Markov Models
por: Keller, Bettina G., et al.
Publicado: (2014)

Direct structural analysis of modified RNA by fluorescent in-line probing
por: Strauss, Benjamin, et al.
Publicado: (2012)

Ligand-modulated folding of the full-length adenine riboswitch probed by NMR and single-molecule FRET spectroscopy
por: Warhaut, Sven, et al.
Publicado: (2017)

Discovery and investigation of natural Diels–Alderases
por: Watanabe, Kenji
Publicado: (2021)

Competitive folding of anti-terminator/terminator hairpins monitored by single molecule FRET
por: Clerte, Caroline, et al.
Publicado: (2013)

Correction to: Discovery and investigation of natural Diels–Alderases
por: Watanabe, Kenji
Publicado: (2021)

Radioactive Phosphorylation of Alcohols to Monitor Biocatalytic Diels-Alder Reactions
por: Nierth, Alexander, et al.
Publicado: (2011)

Artificial Diels–Alderase based on the transmembrane protein FhuA
por: Osseili, Hassan, et al.
Publicado: (2016)

Complete RNA inverse folding: computational design of functional hammerhead ribozymes
por: Dotu, Ivan, et al.
Publicado: (2014)

Secondary structure encodes a cooperative tertiary folding funnel in the Azoarcus ribozyme
por: Mustoe, Anthony M., et al.
Publicado: (2016)

Watching ion-driven kinetics of ribozyme folding and misfolding caused by energetic and topological frustration one molecule at a time
por: Hori, Naoto, et al.
Publicado: (2023)

Molecular Basis for Two Stereoselective Diels–Alderases that Produce Decalin Skeletons
por: Fujiyama, Keisuke, et al.
Publicado: (2021)

Increased Diels-Alderase Activity through Foldit Player Guided Backbone Remodeling
por: Eiben, Christopher B., et al.
Publicado: (2012)

A small ribozyme with dual-site kinase activity
por: Biondi, Elisa, et al.
Publicado: (2012)

SHAPE probing pictures Mg(2+)-dependent folding of small self-cleaving ribozymes
por: Gasser, Catherina, et al.
Publicado: (2018)

Assembly and dynamics of the U4/U6 di-snRNP by single-molecule FRET
por: Hardin, John W., et al.
Publicado: (2015)

Recent advances in the chemo-biological characterization of decalin natural products and unraveling of the workings of Diels–Alderases
por: Watanabe, Kenji, et al.
Publicado: (2022)

Discovery and characterization of a terpene biosynthetic pathway featuring a norbornene-forming Diels-Alderase
por: Sun, Zuodong, et al.
Publicado: (2022)

Exploring the energy landscape of a SAM-I riboswitch
por: Manz, Christoph, et al.
Publicado: (2021)

In vitro selection of a 5′-purine ribonucleotide transferase ribozyme
por: Kang, Taek Jin, et al.
Publicado: (2007)

In vitro evolution of distinct self-cleaving ribozymes in diverse environments
por: Popović, Milena, et al.
Publicado: (2015)

Hairpin ribozyme-antisense RNA constructs can act as molecular lassos
por: Dallas, Anne, et al.
Publicado: (2008)

Examination of the structural and functional versatility of glmS ribozymes by using in vitro selection
por: Link, Kristian H., et al.
Publicado: (2006)

A novel structural rearrangement of hepatitis delta virus antigenomic ribozyme
por: Nehdi, Atef, et al.
Publicado: (2007)

Molecular crowding overcomes the destabilizing effects of mutations in a bacterial ribozyme
por: Lee, Hui-Ting, et al.
Publicado: (2015)

The case for a Diels-Alderase-free, bis-pericyclic, [4+2] dimerization in the biosynthesis of (±)-paracaseolide A
por: Wang, Tao, et al.
Publicado: (2015)

Additional roles of a peripheral loop–loop interaction in the Neurospora VS ribozyme
por: DeAbreu, Diane M., et al.
Publicado: (2011)

Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression
por: Shen, Shensi, et al.
Publicado: (2015)

New tools provide a second look at HDV ribozyme structure, dynamics and cleavage
por: Kapral, Gary J., et al.
Publicado: (2014)

A manganese-dependent ribozyme in the 3′-untranslated region of Xenopus Vg1 mRNA
por: Kolev, Nikolay G., et al.
Publicado: (2008)

Thermodynamics and kinetics for base-pair opening in the P1 duplex of the Tetrahymena group I ribozyme
por: Lee, Joon-Hwa, et al.
Publicado: (2007)

Single-molecule force spectroscopy of the add adenine riboswitch relates folding to regulatory mechanism
por: Neupane, Krishna, et al.
Publicado: (2011)

The structural stabilization of the κ three-way junction by Mg(II) represents the first step in the folding of a group II intron
por: Donghi, Daniela, et al.
Publicado: (2013)

Rational engineering of the Neurospora VS ribozyme to allow substrate recognition via different kissing-loop interactions
por: Lacroix-Labonté, Julie, et al.
Publicado: (2016)

Fluorescent probing for RNA molecules by an unnatural base-pair system
por: Kimoto, Michiko, et al.
Publicado: (2007)

Selection of a novel class of RNA–RNA interaction motifs based on the ligase ribozyme with defined modular architecture
por: Ohuchi, Shoji P., et al.
Publicado: (2008)

The poly(A) site sequence in HDV RNA alters both extent and rate of self-cleavage of the antigenomic ribozyme
por: Brown, Abigail L., et al.
Publicado: (2008)

Contributions and competition of Mg(2+) and K(+) in folding and stabilization of the Twister ribozyme
por: Kognole, Abhishek A., et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_vu2q5c1gaspdego4asihgmvvec