USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products

We present a method that allows simultaneous fusion and cloning of multiple PCR products in a rapid and efficient manner. The procedure is based on the use of PCR primers that contain a single deoxyuridine residue near their 5′ end. Treatment of the PCR products with a commercial deoxyuridine-excisi...

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Detalles Bibliográficos
Autores principales: Geu-Flores, Fernando, Nour-Eldin, Hussam H., Nielsen, Morten T., Halkier, Barbara A.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1874642/
https://www.ncbi.nlm.nih.gov/pubmed/17389646
http://dx.doi.org/10.1093/nar/gkm106

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1874642/
https://www.ncbi.nlm.nih.gov/pubmed/17389646
http://dx.doi.org/10.1093/nar/gkm106

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