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USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products
We present a method that allows simultaneous fusion and cloning of multiple PCR products in a rapid and efficient manner. The procedure is based on the use of PCR primers that contain a single deoxyuridine residue near their 5′ end. Treatment of the PCR products with a commercial deoxyuridine-excisi...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2007
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1874642/ https://www.ncbi.nlm.nih.gov/pubmed/17389646 http://dx.doi.org/10.1093/nar/gkm106 |