Selection strategy and the design of hybrid oligonucleotide primers for RACE-PCR: cloning a family of toxin-like sequences from Agelena orientalis

BACKGROUND: the use of specific but partially degenerate primers for nucleic acid hybridisations and PCRs amplification of known or unknown gene families was first reported well over a decade ago and the technique has been used widely since then. RESULTS: here we report a novel and successful select...

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Detalles Bibliográficos
Autores principales: Pan, Zhensheng, Barry, Richard, Lipkin, Alexey, Soloviev, Mikhail
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1876241/
https://www.ncbi.nlm.nih.gov/pubmed/17498297
http://dx.doi.org/10.1186/1471-2199-8-32

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1876241/
https://www.ncbi.nlm.nih.gov/pubmed/17498297
http://dx.doi.org/10.1186/1471-2199-8-32

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