Carbodiimide-mediated cross-linking of RNA to nylon membranes improves the detection of siRNA, miRNA and piRNA by northern blot

The northern blot, or RNA gel blot, is a widely used method for the discovery, validation and expression analysis of small regulatory RNA such as small interfering RNA (siRNA), microRNA (miRNA) and piwi-interacting RNA (piRNA). Although it is straightforward and quantitative, the main disadvantage o...

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Detalles Bibliográficos
Autores principales: Pall, Gurman Singh, Codony-Servat, Carles, Byrne, Jane, Ritchie, Leigh, Hamilton, Andrew
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1885651/
https://www.ncbi.nlm.nih.gov/pubmed/17405769
http://dx.doi.org/10.1093/nar/gkm112

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1885651/
https://www.ncbi.nlm.nih.gov/pubmed/17405769
http://dx.doi.org/10.1093/nar/gkm112

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