R.KpnI, an HNH superfamily REase, exhibits differential discrimination at non-canonical sequences in the presence of Ca(2+) and Mg(2+)

KpnI REase recognizes palindromic sequence, GGTAC↓C, and forms complex in the absence of divalent metal ions, but requires the ions for DNA cleavage. Unlike most other REases, R.KpnI shows promiscuous DNA cleavage in the presence of Mg(2+). Surprisingly, Ca(2+) suppresses the Mg(2+)-mediated promisc...

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Detalles Bibliográficos
Autores principales: Saravanan, Matheshwaran, Vasu, Kommireddy, Kanakaraj, Radhakrishnan, Rao, Desirazu N., Nagaraja, Valakunja
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1885652/
https://www.ncbi.nlm.nih.gov/pubmed/17430971
http://dx.doi.org/10.1093/nar/gkm114

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1885652/
https://www.ncbi.nlm.nih.gov/pubmed/17430971
http://dx.doi.org/10.1093/nar/gkm114

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