Flow cytometric analysis of DNA binding and cleavage by cell surface-displayed homing endonucleases

LAGLIDADG homing endonucleases (LHEs) cleave 18–24 bp DNA sequences and are promising enzymes for applications requiring sequence-specific DNA cleavage amongst genome-sized DNA backgrounds. Here, we report a method for cell surface display of LHEs, which facilitates analysis of their DNA binding and...

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Detalles Bibliográficos
Autores principales: Volná, Petra, Jarjour, Jordan, Baxter, Sarah, Roffler, Steve R., Monnat, Raymond J., Stoddard, Barry L., Scharenberg, Andrew M.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1885675/
https://www.ncbi.nlm.nih.gov/pubmed/17426121
http://dx.doi.org/10.1093/nar/gkm182

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1885675/
https://www.ncbi.nlm.nih.gov/pubmed/17426121
http://dx.doi.org/10.1093/nar/gkm182

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