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Flow cytometric analysis of DNA binding and cleavage by cell surface-displayed homing endonucleases

LAGLIDADG homing endonucleases (LHEs) cleave 18–24 bp DNA sequences and are promising enzymes for applications requiring sequence-specific DNA cleavage amongst genome-sized DNA backgrounds. Here, we report a method for cell surface display of LHEs, which facilitates analysis of their DNA binding and...

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Detalles Bibliográficos
Autores principales:	Volná, Petra, Jarjour, Jordan, Baxter, Sarah, Roffler, Steve R., Monnat, Raymond J., Stoddard, Barry L., Scharenberg, Andrew M.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2007
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1885675/ https://www.ncbi.nlm.nih.gov/pubmed/17426121 http://dx.doi.org/10.1093/nar/gkm182

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