Cargando…
Hemi-nested touchdown PCR combined with primer-template mismatch PCR for rapid isolation and sequencing of low molecular weight glutenin subunit gene family from a hexaploid wheat BAC library
BACKGROUND: Hexaploid wheat (Triticum aestivum L.) possesses a large genome that contains 1.6 × 10(10 )bp of DNA. Isolation of a large number of gene sequences from complex gene families with a high level of gene sequence identity from genomic DNA is therefore difficult and time-consuming. Bacterial...
| Autores principales: | , |
|---|---|
| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2007
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1885816/ https://www.ncbi.nlm.nih.gov/pubmed/17480230 http://dx.doi.org/10.1186/1471-2156-8-18 |