Vectors for expression of proteins with single or combinatorial fluorescent protein and tandem affinity purification tags in Dictyostelium

We constructed a series of expression vectors for purification of native proteins and protein complexes in Dictyostelium. Protein purification is achieved by either a C-terminal or N-terminal fusion of the protein of choice to the tandem affinity purification (TAP) tag. The TAP tag consists of a pro...

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Detalles Bibliográficos
Autores principales: Meima, Marcel E., Weening, Karin E., Schaap, Pauline
Formato: Texto
Lenguaje:English
Publicado: Academic Press 2007
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1885977/
https://www.ncbi.nlm.nih.gov/pubmed/17296313
http://dx.doi.org/10.1016/j.pep.2007.01.001

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1885977/
https://www.ncbi.nlm.nih.gov/pubmed/17296313
http://dx.doi.org/10.1016/j.pep.2007.01.001

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