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Comparison of RNAi efficiency mediated by tetracycline-responsive H1 and U6 promoter variants in mammalian cell lines

Conditional expression of short hairpin RNAs (shRNAs) to knock down target genes is a powerful tool to study gene function. The most common inducible expression systems are based on tetracycline-regulated RNA polymerase III promoters. During the last years, several tetracycline-inducible U6 and H1 p...

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Detalles Bibliográficos
Autores principales: Henriksen, Jørn R., Løkke, Cecilie, Hammerø, Martin, Geerts, Dirk, Versteeg, Rogier, Flægstad, Trond, Einvik, Christer
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1888813/
https://www.ncbi.nlm.nih.gov/pubmed/17426119
http://dx.doi.org/10.1093/nar/gkm193