Recovery of bisulfite-converted genomic sequences in the methylation-sensitive QPCR

Many methods for the detection of genomic DNA methylation states have appeared. Currently, nearly all such methods employ bisulfite-mediated deamination of denatured DNA. While this treatment effectively deaminates cytosines to uracils, leaving most 5-methylcytosines intact, it also introduces abasi...

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Detalles Bibliográficos
Autores principales: Munson, Kristofer, Clark, Jarrod, Lamparska-Kupsik, Katarzyna, Smith, Steven S.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1888819/
https://www.ncbi.nlm.nih.gov/pubmed/17439964
http://dx.doi.org/10.1093/nar/gkm055

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1888819/
https://www.ncbi.nlm.nih.gov/pubmed/17439964
http://dx.doi.org/10.1093/nar/gkm055

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