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Recovery of bisulfite-converted genomic sequences in the methylation-sensitive QPCR

Many methods for the detection of genomic DNA methylation states have appeared. Currently, nearly all such methods employ bisulfite-mediated deamination of denatured DNA. While this treatment effectively deaminates cytosines to uracils, leaving most 5-methylcytosines intact, it also introduces abasi...

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Detalles Bibliográficos
Autores principales:	Munson, Kristofer, Clark, Jarrod, Lamparska-Kupsik, Katarzyna, Smith, Steven S.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2007
Materias:	Molecular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1888819/ https://www.ncbi.nlm.nih.gov/pubmed/17439964 http://dx.doi.org/10.1093/nar/gkm055

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