Regulation of B family DNA polymerase fidelity by a conserved active site residue: characterization of M644W, M644L and M644F mutants of yeast DNA polymerase ε

To better understand the functions and fidelity of DNA polymerase ε (Pol ε), we report here on the fidelity of yeast Pol ε mutants with leucine, tryptophan or phenylalanine replacing Met644. The Met644 side chain interacts with an invariant tyrosine that contacts the sugar of the incoming dNTP. M644...

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Detalles Bibliográficos
Autores principales: Pursell, Zachary F., Isoz, Isabelle, Lundström, Else-Britt, Johansson, Erik, Kunkel, Thomas A.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1888828/
https://www.ncbi.nlm.nih.gov/pubmed/17452367
http://dx.doi.org/10.1093/nar/gkm132

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1888828/
https://www.ncbi.nlm.nih.gov/pubmed/17452367
http://dx.doi.org/10.1093/nar/gkm132

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