Use of a novel Förster resonance energy transfer method to identify locations of site-bound metal ions in the U2–U6 snRNA complex

U2 and U6 snRNAs pair to form a phylogenetically conserved complex at the catalytic core of the spliceosome. Interactions with divalent metal ions, particularly Mg(II), at specific sites are essential for its folding and catalytic activity. We used a novel Förster resonance energy transfer (FRET) me...

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Detalles Bibliográficos
Autores principales: Yuan, Faqing, Griffin, Laura, Phelps, LauraJane, Buschmann, Volker, Weston, Kenneth, Greenbaum, Nancy L.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1888832/
https://www.ncbi.nlm.nih.gov/pubmed/17430967
http://dx.doi.org/10.1093/nar/gkm134