Expression, purification, crystallization and preliminary crystallographic analysis of MxiH, a subunit of the Shigella flexneri type III secretion system needle

A monodisperse truncation mutant of MxiH, the subunit of the needle from the Shigella flexneri type III secretion system (TTSS), has been overexpressed and purified. Crystals were grown of native and selenomethionine-labelled MxiH(CΔ5) and diffraction data were collected to 1.9 Å resolution. The cry...

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Detalles Bibliográficos
Autores principales: Deane, Janet E., Cordes, Frank S., Roversi, Pietro, Johnson, Steven, Kenjale, Roma, Picking, William D., Picking, Wendy L., Lea, Susan M., Blocker, Ariel
Formato: Texto
Lenguaje:English
Publicado: International Union of Crystallography 2006
Materias:
Crystallization Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1894743/
https://www.ncbi.nlm.nih.gov/pubmed/16511329
http://dx.doi.org/10.1107/S1744309106006555

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1894743/
https://www.ncbi.nlm.nih.gov/pubmed/16511329
http://dx.doi.org/10.1107/S1744309106006555

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